Lehrstuhl Biochemie der Ernährung

Publikationen

 

Originalarbeiten

 

2013 Neuschäfer-Rube F, Lieske S, Kuna M, Henkel J, Perry R, Erion D, Pesta D, von Loeffelholz C, Willmes D, Tolkachov A, Brachs S, Schupp M, Pfeiffer A, Pathe-Neuschäfer-Rube A, Shulman G, Püschel GP and Birkenfeld, A (2013)
The Mammalian INDY Homolog is Induced by CREB in a Rat Model of Type 2 Diabetes
Diabetes 2013 accepted
   
  Neuschäfer-Rube F, Pathe-Neuschäfer-Rube A, Hippenstiel S, Kracht M, Püschel GP (2013)
NF-KappaB-dependent IL-8 induction by prostaglandin E2 receptors EP1 and EP4.
Br J Pharmacol . 2013 168:704-717
   
2012  
  Henkel J, Frede K, Schanze N, Vogel H, Schürmann A, Spruss A, Bergheim I, Püschel GP.(2012)
Stimulation of fat accumulation in hepatocytes by PGE(2)-dependent repression of hepatic lipolysis, ß-oxidation and VLDL-synthesis.
Lab Invest . 2012 92:1597-606.
   
  Spruss A, Henkel J, Kanuri G, Blank D, Püschel GP, Bischoff SC, Bergheim I (2012)
Female mice are more susceptible to non-alcoholic fatty liver disease: sex-specific regulation of the hepatic AMP-activated protein kinase - plasminogen activator inhibitor 1-cascade but not the hepatic endotoxin response
Molmed 2012 18:1346-1355
   
  Hesse D, Jaschke A, Kanzleiter T, Witte N, Augustin R, Hommel A, Püschel GP, Petzke KJ, Joost HG, Schupp M, Schürmann A (2012)
GTPase ARFRP1 is essential for normal hepatic glycogen storage and insulin-like growth factor 1 secretion.
Mol Cell Biol .2012 32:4363-74
   
  Szymanski KV, Toennies M, Becher A, Fatykhova D, N'guessan PD, Gutbier B, Klauschen F, Neuschaefer-Rube F, Schneider P, Rueckert J, Neudecker J, Bauer TT, Dalhoff K, Drömann D, Gruber AD, Kershaw O, Temmesfeld-Wollbrueck B, Suttorp N, Hippenstiel S, Hocke AC. (2012)
Streptococcus pneumoniae-induced regulation of cyclooxygenase-2 in human lung tissue.
Eur Respir J . 2012 40(6):1458-67
   
2011  
  Henkel J, Gärtner D, Dorn C, Hellerbrand C, Schanze N, Elz SR, Püschel GP.(2011)
Oncostatin M produced in Kupffer cells in response to PGE2: possible contributor to hepatic insulin resistance and steatosis
Lab Invest . 2011 91:1107-17
   
2009  
  Wieneke N , Neuschäfer-Rube F , Bode LM , Kuna M , Andres J , Carnevali LC Jr , Hirsch-Ernst KI , Püschel GP .(2009)
Synergistic acceleration of thyroid hormone degradation by phenobarbital and the PPAR alpha agonist WY14643 in rat hepatocytes.
Toxicol Appl Pharmacol. 2009 240;99-107
   
 

Henkel J, Neuschäfer-Rube F, Pathe-Neuschäfer-Rube A, Püschel GP (2009)
Aggravation by prostaglandin E2 of interleukin-6-dependent insulin resistance in hepatocytes.
Hepatology . 2009 50;781-90

   
2007  
 

Wieneke N, Hirsch-Ernst KI, Kuna M, Kersten S, Püschel GP (2008)
PARalpha-dependent induction of the energy homeostasis-regulating nuclear receptor NR1i3 (CAR) in rat hepatocytes: potential role in starvation adaptation.
FEBS Lett . 2007 581;5617-26..

   
2005  
  Giening G, Jurgensen J, Eichhorst J, Furkert J, Beyermann M, Neuschafer-Rube F, Rosenthal W, Zidek W, van der Giet M and Oksche A (2005)
Arrestin-Independent Internalization and Recycling of the Urotensin Receptor Contribute to Long-Lasting Urotensin II-Mediated Vasoconstriction.
Circ Res. 2005 Sep 30;707-15. (abstract)
   
  Neuschäfer-Rube F, Hermosilla R, Kuna M, Pathe-Neuschäfer-Rube A, Schülein R, Puschel GP. (2005)
A Ser/Thr cluster within the C-terminal domain of the rat prostaglandin receptor EP3alpha is essential for agonist-induced phosphorylation, desensitization and internalization.
Br J Pharmacol. 2005 Aug 145;1132-42. (abstract)
   
  Pathe-Neuschäfer-Rube A, Neuschäfer-Rube F and Püschel GP. (2005)
Role of the ERC-motif in the proximal part of the second intracellular loop and the C-terminal domain of the human prostaglandin F2alpha receptor (FP receptor) in G-protein coupling control.
Biochem J. 2005 Jan14; 388:317-324 (abstract)
   
2004  
  Püschel GP. (2004)
Control of hepatocyte metabolism by sympathetic and parasympathetic hepatic nerves.
Anat Rec. 280A:854-867. (abstract)
   
  Neuschäfer-Rube F, Hermosilla R, Rehwald M, Rönnstrand L, Schülein R, Wernstedt C, Püschel GP. (2004)
Identification of a Ser/Thr cluster in the C-terminal domain of the human prostaglandin receptor EP4 that is essential for agonist-induced beta-arrestin1 recruitment but differs from the apparent principal phosphorylation site.
Biochem J. 379:573-85 (abstract)
   
2003  
  Boer U, Fennekohl A, Püschel GP. (2003)
Sensitization by interleukin-6 of rat hepatocytes to tumor necrosis factor alpha-induced apoptosis.
J Hepatol. 38:728-35. (abstract)
   
  Neuschäfer-Rube F, Engemaier E, Koch S, Boer U, Püschel GP. (2003)
Identification by site-directed mutagenesis of amino acids contributing to ligand-binding specificity or signal transduction properties of the human FP prostanoid receptor.
Biochem J. 371:443-9. (abstract)
   
2002  
  Fennekohl A, Sugimoto Y, Segi E, Maruyama T, Ichikawa A, Püschel GP. (2002)
Contribution of the two Gs-coupled PGE2-receptors EP2-receptor and EP4-receptor to the inhibition by PGE2 of the LPS-induced TNFalpha-formation in Kupffer cells from EP2-or EP4-receptor-deficient mice. Pivotal role for the EP4-receptor in wild type Kupffer cells.
J Hepatol. 36:328-34. (abstract)
   
2000  
  Neuschäfer-Rube F, Möller U, Püschel GP. (2000)
Structure of the 5'-flanking region of the rat prostaglandin F(2alpha) receptor gene and its transcriptional control functions in hepatocytes
Biochem Biophys Res Commun. 278:278-85. (abstract)
   
  Boer U, Neuschäfer-Rube F, Möller U, Püschel GP. (2000)
Requirement of N-glycosylation of the prostaglandin E2 receptor EP3beta for correct sorting to the plasma membrane but not for correct folding.
Biochem J. 350:839-847. (abstract)
   
  Fennekohl A, Lucas M, Püschel GP. (2000)
Induction by interleukin 6 of G(s)-coupled prostaglandin E(2) receptors in rat hepatocytes mediating a prostaglandin E(2)-dependent inhibition of the hepatocyte's acute phase response.
Hepatology. 31:1128-34. (abstract)
   
1999  
  Schieferdecker HL, Pestel S, Püschel GP, Götze O, Jungermann K (1999)
Increase by anaphylatoxin C5a of glucose output in perfused rat liver via prostanoids derived from nonparenchymal cells: direct action of prostaglandins and indirect action of thromboxane A(2) on hepatocytes.
Hepatology. 30:454-61. (abstract)
   
  Neuschäfer-Rube F, Oppermann M, Möller U, Boer U, Püschel GP (1999)
.Agonist-induced phosphorylation by G protein-coupled receptor kinases of the EP4 receptor carboxyl-terminal domain in an EP3/EP4 prostaglandin E(2) receptor hybrid.
Mol Pharmacol. 56:419-28. (abstract)
   
  Fennekohl A, Schieferdecker HL, Jungermann K, Püschel GP (1999)
Differential Expression of Prostanoid Receptors in Hepatocytes, Kupffer Cells, Sinusoidal Endothelial Cells and Stellate Cells of Rat Liver.
J Hepatology 30:38-47. (abstract)
   
  Neuschäfer-Rube F, Möller U, Oppermann M, Püschel GP (1999)
Agonist-induced sequestration of an EP3/EP4-prostaglandin E2 receptor hybrid by GRK-dependent phosphorylation of the EP4-R C-terminal domain.
Mol Pharmacol 56:419-28. (abstract)
   
  Rehwald M, Neuschäfer-Rube F, de Vries C, Püschel GP (1999)
Possible role for ligand binding of histidine 81 in the second transmembrane domain of the rat prostaglandin F2a receptor
FEBS Lett 443:357-362. (abstract)
   
1998  
  Schieferdecker HL, Pestel S, Rothermel E, Püschel GP, Götze O, Jungermann K (1998)
Stimulation by anaphylatoxin C5a of glycogen phosphorylase in rat hepatocytes via prostanoid release from hepatic stellate cells but not sinusoidal endothelial cells. FEBS Lett 434:245-250. (abstract)
   
1997  
  Boie Y, Stocco R, Sawyer N, Slipetz MD, Ungrin M, Neuschäfer-Rube F, Püschel GP, Metters KM, Abramowitz M (1997)
Molecular cloning and characterization of the four rat prostaglandin E2 prostanoid receptor subtypes.
Eur J Pharmacol 340:227-241 (abstract)
   
 
Neuschäfer-Rube F, Hänecke K, Püschel GP (1997)
The C-terminal domain of the human EP4 receptor confers agonist-induced receptor desensitization in a receptor hybrid with the rat EP3ß receptor.
FEBS Lett 415: 119-124 (abstract)
   
 
Neuschäfer-Rube F, Hänecke K, Blaschke V, Jungermann K, Püschel GP (1997)
The C-terminal domain of the Gs-coupled EP4 receptor confers agonist-dependent coupling control to Gi but no coupling to Gs in a receptor hybrid with the Gi-coupled EP3 receptor.
FEBS-Lett. 401:185-190. (abstract)
   
1996  
 
Blaschke V, Jungermann K, Püschel GP (1996)
Exclusive expression of the Gs-linked prostaglandin E2 receptor subtype 4 mRNA in Jurkat and KM3 cells and coexpression of the subtype 4 and 2 mRNA in U937 cells.
FEBS Lett 394: 39-43 (abstract)
   
 
Püschel GP, Nolte A, Schieferdecker HL, Rothermel E, Götze O, Jungermann K (1996)
Attenuation of anaphylatoxin C3a and C5a but not nerve stimulation or noradrenaline-mediated increase in glycogenolysis and reduction of flow in perfused rat liver by selective Kupffer cell elimination with gadolinium chloride.
Hepatology 24: 685-690. (abstract)
   
 
Stümpel F, Kucera T, Bazotte R, Püschel GP (1996)
Loss of regulation by sympathetic hepatic nerves of liver metabolism and hemodynamics in chronically streptozotocin diabetic rats.
Diabetologia 39: 161-165. (abstract)
   
1995  
 
Hespeling U, Jungermann K, Püschel GP (1995)
Feedback-inhibition of glucagon-stimulated glycogenolysis in hepatocyte/Kupffer cell co-cultures by glucagon-elicited prostaglandin production in Kupffer cells.
Hepatology 22: 1577-1583. (abstract)
   
 
Hespeling U, Püschel GP, Götze O, Jungermann K, Zwirner J (1995)
Stimulation of hepatocyte glycogen phosphorylase via prostaglandin release from Kupffer cells in hepatocyte/Kupffer cell co-culutres by recombinant rat but not native human C5a
FEBS-Lett 372: 108-112 (abstract)
   
 

Püschel GP, Christ B (1994)
Inhibition by PGE2 of glucagon-induced increase in phosphoenolpyruvate carboxykinase mRNA and acceleration of mRNA degradation in cultured rat hepatocytes.
FEBS Lett. 351: 353-356 (abstract)

   
  Neuschäfer-Rube F, De Vries C. Hänecke K, Jungermann K, Püschel GP (1994)
Molecular cloning and expression of a prostaglandin E2 receptor of the EP3ß subtype from rat hepatocytes.
FEBS Lett. 351: 119-122. (abstract)
   
  Watanabe Y, Püschel GP, Gardemann A, Jungermann K (1994)
Pre- and proximal intrasinusoidal confluence of hepatic artery and portal vein in rat liver: Functional evidence by orthograde and retrograde bivascular perfusion.
Hepatology 19: 1198-1207. (abstract)

 

Buchbeiträge

 

1999  
Püschel GP, Segi E, Fennekohl A, Sugimoto Y, Ikegami R, Katsuyama M, Ichikawa A. (1999)
Role of the EP2 and EP4 receptor in the prostaglandin E2-mediated feedback inhibition of lipopolysaccharide-induced TNFa formation in mouse Kupffer cells.
In Cells of the hepatic sinusoid 7, Wisse E, Knook DL, Frazer R eds. Kupffer cell Foundation, Leiden, The Netherlands
   
1997
Schieferdecker HL, Püschel GP, Rothermel E, Timmermann A, Jungermann K (1997)
The thromboxane receptor antagonist daltroban inhibits glucose output and increases prostanoid overflow induced by anaphylatoxins in perfused rat liver but not in isolated Kupffer cells.
In Cells of the hepatic sinusoid, 6; Kupffer Cell Foundation, Rijswijk The Netherlands. pp 357-359
Püschel GP, Schieferdecker HL, Fennekohl A, Timmermann A, Jungermann K (1997)
Differential Expression of Prostanoid Receptors in Kupffer Cells, Sinusoidal Endothelial Cells and Hepatocytes.
In Cells of the hepatic sinusoid, 6; Kupffer Cell Foundation, Rijswijk The Netherlands. pp 134-137
   
1996
Püschel GP, Neuschäfer-Rube F, de Vries C, Hänecke K, Nolte A, Kirchner C, Schröder A, Schestag F, Athari A, Jungermann K (1996)
Caracterization and molecular cloning of hepatocyte prostaglandin receptors possibly involved in the nerve stimulation-dependent increase in hepatic glucose output.
In: Liver innervation, Shimazu T, ed. John Libbey & Comp. pp 87-94
   
1995
Hespeling U, Jungermann K, Püschel GP (1995)
Glucagon-stimulated prostaglandin formation in Kupffer cells: A step in the feedback inhibition of glucagon-stimulated glycogenolysis in hepatocytes.
In Cells of the hepatic sinusoid, 4; Kupffer Cell Foundation, Rijswijk The Netherlands, pp171-173

 

Reviews (auf Einladung)

 

1996
Jungermann K, Püschel GP, Gardemann A, Stümpel F (1996)
Control of liver metabolism and hemodynamics by hepatic nerves.
In: Liver innervation, Shimazu T, ed. John Libbey & Comp. pp 105-113.
   
1994
Püschel GP, Jungermann K (1994)
Integration of function in the hepatic acinus: neural and intercellular communication.
Progr Liver Dis 12: 19-46.

 

Abstracts

 

2004  
 

Neuschäfer-Rube F, Hermosilla R, Kuna M, Pathe-Neuschäfer-Rube A,Püschel GP (2004)
Agonist-induced desensitization of rat prostaglandin EP3 receptor isoforms.
Naunyn Schmied Arch Pharmacol 369:R20

   
  Pathe-Neuschäfer-Rube A, Neuschäfer-Rube F, Püschel GP (2004)
G protein coupling control by the ERC motif in the proximal part of the second intracellular loop and the C-terminal domain of the human prostaglandin F 2 a receptor (FP-R).
Naunyn Schmied Arch Pharmacol 369:R21
   
2003  
 
Pathe-Neuschäfer-Rube A, Neuschäfer-Rube F, Püschel GP. (2003)
A conserved glutamate residue in the second intracellular loop and the C-terminal domain of the human prostaglandin F2a -receptor confer agonist-independent coupling-control to Gq.
Abstracts 8 th International Dahlem symposium on “Cellular signal recognition and tranduction” P17
2002
Neuschäfer-Rube F, Böer U, Rehwald M, Püschel GP. (2002)
Mapping of serine residues in the C-terminal domain of the human EP4 receptor relevant for agonist-induced phosphorylation and sequestration.
Naunyn Schmied Arch Pharmacol 365:R20
   
2001
Engemaier E, Neuschäfer-Rube F, Püschel GP. (2001)
Characterization of single key amino acid involved in ligand binding and G protein coupling of the human prostaglandin F 2 a -receptor.
Naunyn Schmied Arch Pharmacol 363:R21
   
 
Neuschäfer-Rube F, Böer U, Rehwald M, Püschel GP. (2001)
Agonist-induced phosphorylation and sequestration of the human prostaglandin E 2 receptor, subtype EP4 - Identification of relevant serine/threonine residues in the C-terminal receptor domain.
Signal Transduction 1:152
2000
 
Engemaier E, Neuschäfer-Rube F, Püschel GP. (2000)
Characterization of the putative F-prostaglandin receptor binding site by site directed mutagenesis based exchange of key amino acids.
Naunyn Schmied Arch Pharmacol Suppl. 362:R22
   
 
Böer U, Neuschäfer-Rube F, Möller U, Püschel GP. (2000)
Requirement of N-glycosylation of the prostaglandin E 2 receptor EP3ß for correct sorting to the plasma membrane.
Naunyn Schmied Arch Pharmacol Suppl. 362:R22
 
Neuschäfer-Rube F, Möller U, Püschel GP. (2000)
Charakterisierung der Struktur und transkriptionellen Funktion des 5'-flankierenden Bereichs des Ratten Prostaglandin F 2 a -Rezeptor Gens.
Z Gastroenterol 38:97.
1999
 
Neuschäfer-Rube F, Möller U, Oppermann M, Püschel GP. (1999)
Agonist-induced sequestration of an EP3/EP4-prostaglandin E 2 receptor hybrid by GRK-dependent phosphorylation of the hEP4-R C-terminal domain.
Eur J Cell Biol 78 Suppl. 49:73.
 
Rehwald M, Neuschäfer-Rube F, Möller U, Püschel GP. (1999)
Possible role for ligand binding of the histidine 81 residue in the second transmembrane domain of the rat prostaglandin F 2 a -receptor.
Naunyn Schmied Arch Pharmacol ;359:R20.
 
Püschel GP, Fennekohl A, Sugimoto Y, Segi E, Ikegami R, Katsuyama M, Ichikawa A (1999)
Bedeutung des EP2 und EP4 Rezeptors für die Prostaglandin E2-vermittelte Rückkopplungshemmung der Lipopolysaccharid-induzierten TNFa -Bildung in Maus Kupffer Zellen.
Z Gastroenterol 37, 18
1998
  Böer U, Neuschäfer-Rube F, Püschel GP (1998)
Function of glycosylation sites and the C-terminal domain of prostaglandin E2 receptors EP3ß and EP4.
Naunyn Schmied Arch Pharmacol 357,R15.
 
  Fennekohl A, Neuschäfer-Rube F, Püschel GP (1998)
Funktioneller und molekularbiologischer Nachweis eines Prostaglandin-Transporters in Rattenhepatocyten.
Z Gastroenterol 36, 60.
   
1997  
  Neuschäfer-Rube F, Hänecke K, Püschel GP (1997)
The C-terminal Domain of the human EP4 receptor confers agonist-induced receptor desensitization in a receptor hybrid with the rat EP3ß receptor.
Eur J Cell Biol 72 Suppl. 43: 77.
   
  Püschel GP, Fennekohl A, Schieferdecker H, Timmermann A, Jungermann K (1997)
Nachweis der differenziellen Expression von Prostanoidrezeptoren des FP, EP2, EP4, TP IP Typs in Hepatocyten, Kupfferzellen und sinusoidalen Endothelzellen der Rattenleber durch semiquantitative RT-PCR.
Z Gastroenterol 35: 62.
   
1996  
  Püschel GP, Neuschäfer-Rube F, de Vries C, Hespeling U, Jungermann K (1996)
Molecular characterization of hepatic prostanoid receptors.
Prostag Leukot Essent Fatty Acids 55, Supp1: 133.
   
  Blaschke V, Jungermann K, Püschel GP (1996)
Characterization of cAMP-elevating prostaglandin E2 receptors on U937, Jurkat and other human mononuclear cell lines.
Eur J Cell Biol 69 Suppl. 42:127.
   
  Neuschäfer-Rube F, Hänecke K, Püschel GP (1996)
A hybrid prostaglandin receptor between the N-terminal main portion of the Gi-coupled EP3 receptor and the C-terminal portion of the Gs-coupled EP4 receptor couples to the Gi protein.
Eur J Cell Biol 69 Suppl. 42:78.
   
  Püschel GP, Nolte A, Schieferdecker H, Rothermel E, Jungermann K (1996)
Hemmung der durch humanes Anaphylatoxin C3a und rekombinantes Ratten C5a nicht aber durch Neurostimulation oder Noradrenalin ausgelösten Steigerung der Glukoseabgabe und Reduktion des Flusses in Kupfferzell-freien perfundierten Rattenlebern.
Z Gastroenterol 34: 54.
   
  de Vries C, Neuschäfer-Rube F, Kietzmann T, Hänecke K, Freimann S, Jungermann K, Püschel GP (1996)
Klonierung, Expression, funktionelle Charakterisierung und intrahepatische Lokalisation eines Hepatocyten Prostaglandin F2a Rezeptors.
Z Gastroenterol 34: 47.
   
  de Vries C, Neuschäfer-Rube F, Hänecke K, Jungermann K, Püschel GP (1996)
Molecular cloning, expression and functional chararacterization of a hepatocyte prostaglandin F2a receptor.
Hepatology 22: 304A.

 

 

Abteilungen

Kontakt

Universität Potsdam
Institut für Ernährungswissenschaft
Arthur-Scheunert-Allee 114-116
14558 Nuthetal
OT Bergholz-Rehbrücke
Tel: 033200/88-5269
Fax: 033200/88-5541
E-Mail: hmahler@uni-potsdam.de

Anfahrt zum Institut

 

Mathematisch-Naturwissenschaftliche Fakultät